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Recombinant Mouse IFN gamma (E. coli)

CAT: 0855-EPT095Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0855-EPT095Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mouse Ifng is a secreted protein which belongs to the type I I (or gamma) interferon family. IFNG is produced by lymphocytes and activated by specific antigens or mitogens. In addition to having antiviral activity, IFNG also has important immunoregulatory functions. It is a potent activator of macrophages and has antiproliferative effects on transformed cells. It can potentiate the antiviral and antitumor effects of the type I interferons. Genetic variation in IFNG is associated with the risk of aplastic anemia (AA) which is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Description
Recombinant Mouse Interferon Gamma is produced by our E.coli expression system and the target gene encoding His23-Cys155 is expressed.
UniProt
P01580
Accession Number
P01580
Endotoxin
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED50 is 1 ng/ml.
Molecular Weight
15.7 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Storage Temperature
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Notes
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in 4mM HCl. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Observed Molecular Weight
14 KDa, reducing conditions
Other Product Names
Ifng; Interferon gamma; IFN-gamma

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