Recombinant 2019-nCoV Helicase (N-6His-MBP)

• Background:

  The non—structural protein 13 (nsp13) of SARS—CoV 2 is a helicase that separates double—stranded RNA or DNA with a 5'—3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.

• Description:

  Recombinant 2019-nCoV Helicase is produced by our E.coli expression system and the target gene encoding Ala5325-Gln5925 is expressed with a 6His, MBP tag at the N-terminus.

• Synonyms:

  SARS-CoV 2 Helicase; SARS-CoV 2 nsp13

• UniProt:

  P0DTD1

• Accession Number:

  P0DTD1

• Purity:

  Greater than 80% as determined by reducing SDS-PAGE.

• Molecular Weight:

  112.8 KDa

• Shipping Conditions:

  The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Notes:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight:

  120 KDa, reducing conditions

• Other Product Names:

  SARS-CoV 2 Helicase; SARS-CoV 2 nsp13

• CAS Number:

  9000-83-3