Recombinant Human IL-1b

• Background:

  IL1B belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells.

• Description:

  Recombinant Human Interleukin-1 Beta is produced by our E.coli expression system and the target gene encoding Ala117-Ser269 is expressed.

• Synonyms:

  Interleukin-1 beta; Catabolin; IL1F2; IL1B

• UniProt:

  P01584

• Accession Number:

  P01584

• Endotoxin:

  Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells (Cat#XCC10) . The ED50 for this effect is 20-100 pg/ml.

• Molecular Weight:

  17.5 KDa

• Shipping Conditions:

  The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Notes:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight:

  17 KDa, reducing conditions

• Other Product Names:

  Interleukin-1 beta; Catabolin; IL1F2; IL1B

• CAS Number:

  9000-83-3