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Recombinant Human Mitotic checkpoint protein BUB3 (BUB3)

CAT: 0399-CSB-EP002884HUj9-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP002884HUj9-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
BUB3
Abbreviation
Recombinant Human BUB3 protein
Gene Name
BUB3
UniProt
O43684
Expression Region
1-328aa
Organism
Homo sapiens (Human)
Target Sequence
MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT
Tag
C-terminal 6xHis-Avi-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Cell Biology
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Bioactivity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
39.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length

UniProtKB · O43684

Mitotic checkpoint protein BUB3

BUB3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43684
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BUB3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A6NJ42, B2R6E7, D3DRE9, O43685
Protein keywords

PTM

AcetylationADP-ribosylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionChromosome partitionMeiosisMitosis

Cellular component

CentromereChromosomeKinetochoreNucleus

Technical term

Proteomics identificationReference proteome

Domain

RepeatWD repeat

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