Recombinant Human T-cell surface glycoprotein CD4 (CD4), partial (Active)

• Product Name Alternative:

  T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4

• Gene Name:

  CD4

• UniProt:

  P01730

• Expression Region:

  26-396aa

• Organism:

  Homo sapiens (Human)

• Tag:

  C-terminal 10xHis-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Immunology

• Endotoxin:

  Less than 1.0 EU/µg as determined by LAL method.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Bioactivity:

  Measured by its binding ability in a functional ELISA.Immobilized Human CD4 at 2 μg/mL can bind Anti-CD4 recombinant antibody (CSB-RA004935MA3HU) .The EC50 is 4.219-4.754 ng/mL.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  42.7 kDa

• References & Citations:

  Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN. Loewenberg M., Verhaar A.P., Bilderbeek J., Marle J.V., Buttgereit F., Peppelenbosch M.P., van Deventer S.J., Hommes D.W. EMBO Rep. 7:1023-1029 (2006)

• Shelf Life:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Transmembrane Domains:

  0TM

• Protein Length:

  Partial

• Target Description:

  KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP