Recombinant Human Wnt3a surrogate (Active)

• Gene Name:

  Wnt3a surrogate

• Expression Region:

  1-306aa

• Organism:

  Homo sapiens (Human)

• Tag:

  C-terminal hFc1-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Endotoxin:

  Less than 1.0 EU/µg as determined by LAL method.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Bioactivity:

  Measured by its ability to induce Topflash reporter activity in HEK293T cells. The ED50 for this effect is 17.76-26.50 ng/mL.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered 25 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.2

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  58.5 kDa

• Shelf Life:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length