NativeFolder Bacterial Culture Medium
| Catalog # | _x000D_K1010004 | _x000D_
| Content | _x000D_NativeFolderTM Mix A_x000D_ _x000D_ NativeFolderTM Mix B_x000D_ _x000D_ Instructions | _x000D_
| Keywords | _x000D_Culture Medium, folding, solubilization, recombinant proteins, soluble protein expression, expression insoluble proteins, purification of soluble protein from e.coli | _x000D_
| Storage | _x000D_Room temperature | _x000D_
| Expression Protocol | _x000D_1- Dissolve NativeFolderTM Mix A in 0.75 L of di-ionized water, stir until completely dissolved and autoclave._x000D_ _x000D_ 2- Dissolve NativeFolderTM Mix B in 0.25 L of di-ionized water, stir until completely dissolved filter sterilize before use (DO NOT AUTOCLAVE)._x000D_ _x000D_ 3- Combine 0.75 L of NativeFolderTM Mix A with 0.25 L of NativeFolder Mix B to make 1.0 L of NativeFolderTM Culture Medium. Follow Steps 4-10. | _x000D_
NativeFolderTM Flyer_x000D_
 | _x000D_
NativeFolderTM Explained (Download Presentation)_x000D_ [embeddoc url="https://moleculardepot.com/wp-content/uploads/2019/03/NativeFolder_data.pdf" download="all"] | _x000D_
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- Packiam KAR, Ramanan RN, Ooi CW, Krishnaswamy L, Tey BT. Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches. Appl Microbiol Biotechnol. 2020 Apr;104(8):3253-3266. _x000D_
- Satheeshkumar PK. Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update. Mol Biotechnol. 2020 Mar;62(3):151-167. _x000D_
- Smolskaya S, Logashina YA, Andreev YA. Escherichia coli Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis. Int J Mol Sci. 2020 Jan 31;21(3). pii: E928. _x000D_
- Lipinski DM. A Comparison of Inducible Gene Expression Platforms: Implications for Recombinant Adeno-Associated Virus (rAAV) Vector-Mediated Ocular Gene Therapy. Adv Exp Med Biol. 2019;1185:79-83. _x000D_
- Powell N, Pantazi E, Pavlidis P, Tsakmaki A, Li K, Yang F, Parker A, Pin C, Cozzetto D, Minns D, Stolarczyk E, Saveljeva S, Mohamed R, Lavender P, Afzali B, Digby-Bell J, Tjir-Li T, Kaser A, Friedman J, MacDonald TT, Bewick GA, Lord GM. Interleukin-22 orchestrates a pathological endoplasmic reticulum stress response transcriptional programme in colonic epithelial cells. Gut. 2020 Mar;69(3):578-590. _x000D_
- Temporini C, Colombo R, Calleri E, Tengattini S, Rinaldi F, Massolini G. Chromatographic tools for plant-derived recombinant antibodies purification and characterization. J Pharm Biomed Anal. 2020 Feb 5;179:112920. _x000D_
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