EasyStep Human BNP (Brain Natriuretic Peptide) ELISA Kit

EasyStep Human BNP (Brain Natriuretic Peptide) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP. After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
• Field of Research: Cardiovascular biology; Neuro science; Hormone metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 1.5h
• Standard: 2000 pg/mL
• Sample Type: Serum, plasma
• Detection Range: 31.25-2000 pg/mL
• Sensitivity: 6.8 pg/mL