CRM-bromoacetate

CAT: 0898-EcoCRM®-Bromoactate-25Size: 25 mgDry Ice: NoHazardous: No
CAT#:0898-EcoCRM®-Bromoactate-25Size:25 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fina Biosolutions now offersCRM197pre-labeled with bromoacetate, allowing for the easy conjugation of thiolated antigens.  FinaBio’s CRM197, EcoCRM®, is labeled with ~15 bromoacetate groups.  The labeled protein is provided frozen, ready to thaw and conjugate to a thiol-containing antigen. Examples of antigens that have been conjugated toCRM-bromoacetateinclude cysteine-peptides, thiol-labeled oligosaccharides, polysaccharides and proteins.  Depending on the nature of the antigen, gel filtration or spin filters can be used to purify the conjugate. CRM-bromoactate ~15 Bromoacetate/CRM1975 mg/mlPBS + 5% sucrose, pH 6.8Store at <-20°C Note:Use within 1 hr after thawing.Note that CRM197will denature in acidic buffers.Care should be taken to keep the conjugation pH between 6.8 and 9 for CRM-bromoacetate.We recommend familiarity with thiol-ether conjugation.See GT Hermanson, Bioconjugate Techniques.

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