Anti-Id3 Antibody

Anti-Id3 Antibody

• Description: Mouse Monoclonal Antibody specific to Id3
• Product Name Alternative: Class B basic helix-loop-helix protein 25, bHLHb25, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, Inhibitor of differentiation 3
• CAS Number: 9007-83-4
• Gene Name: ID3
• NCBI Gene ID: ID3
• UniProt: Q02535
• Cellular Locus: Nucleus.
• Host: Mouse
• Reactivity: Human, Mouse
• Immunogen: Mouse Id3 gst-fusion protein (aa 1-119) expressed in E. coli.
• Target Antigen: DNA-binding protein inhibitor ID-3
• Target: Id3
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, ICC/IF, IP
• Field of Research: Neuroscience
• Purification Method: Purified by Protein A affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium which is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. {PubMed:8437843}.
• Additionnal Information: Immunoblotting and Immuno- precipitation: use at 1-5 ug/mL.
  
  Positive controls: Id3 transfected Sol8 muscles cells and recombinant fusion protein.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
• Specificity: This antibody recognizes human and mouse Id3.
• Formulation: 10 mM PBS, pH 7.4.
• Buffer pH: pH 7.4
• Target Background: Id3 is a 14 kD protein and a member of the Id family of helix-loop-helix (HLH) proteins. Id proteins are believed to be dominant negative regulators of HLH involved in cell differentiation and implicated in the control of cell growth, cellular differentiation, apoptosis, and carcinogenesis.