Multiplex Assay Kit for Chimerin 1 (CHN1)

CAT: 0338-TRV-062688Size: 96 TestsDry Ice: NoHazardous: No

1 / 1

CAT#:0338-TRV-062688Size:96 Tests

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

UniProt

P15882

Applications

FLIA Kit for Antigen Detection.

Assay Principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Assay Protocol

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37° C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37° C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37° C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Assay Performance Time

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Detection Range

0.02 - 20 ng/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level of the target, etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level of the target, etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/meanX100, Intra-Assay: CV<10%, Inter-Assay: CV<12%

Sensitivity

The minimum detectable dose of this kit is typically less than 0.007ng/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of the target, etc. by FLIA (Flow Luminescence Immunoassay) .
No significant cross-reactivity or interference between the target, etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Method

Double-Antibody Sandwich

Organism Species

Homo sapiens (Human)

UniProtKB · P15882

N-chimaerin

CHIN_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P15882
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CHN1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: A8K1M6, B3KNU6, B4DV19, Q53SD6, Q53SH5, Q96FB0

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Disease variant

Molecular function

GTPase activation

Ligand

Metal-bindingZinc

Biological process

Neurogenesis

Domain

SH2 domainZinc-finger

Related Products

Catalog	Name
TRV-054838-01	ELISA Kit for Chimerin 1 (CHN1)
TRV-029043-01	Recombinant Chimerin 1 (CHN1)
TRV-017464-01	Polyclonal Antibody to Chimerin 1 (CHN1)
TRV-062987	Multiplex Assay Kit for Spondin 1 (SPON1)
TRV-062828	Multiplex Assay Kit for Pannexin 1 (PANX1)
TRV-061982	Multiplex Assay Kit for Secernin 1 (SCRN1)
TRV-061773	Multiplex Assay Kit for Gastrokine 1 (GKN1)
TRV-060627	Multiplex Assay Kit for Anoctamin 1 (ANO1)
TRV-061965	Multiplex Assay Kit for Tensin 1 (TNS1)
TRV-061592	Multiplex Assay Kit for Atrophin 1 (ATN1)

Alternative Products

Catalog	Name
abx132823-01	Chimerin 1 (CHN1) Antibody
SIPE950Hu01-01	Recombinant Chimerin 1 (CHN1)
CAU36623-01	Chimerin 1 (CHN1) Polyclonal Antibody
abx652891-01	Human Chimerin 1 (CHN1) Protein
MBS2170104-01	Polyclonal Antibody to Chimerin 1 (CHN1)
MBS2710292-01	Human Chimerin 1 (CHN1) ELISA Kit
KBH8892	GENLISA Human Chimerin 1 (CHN1) ELISA
JOT-EK2509Hu-01	Human Chimerin 1,CHN1 ELISA KIT
477961	CHN1 (N-Chimaerin, Chimerin (Chimaerin) 1)
KBH2509	GENLISA™ Human Chimerin 1 (CHN1) ELISA