Multiplex Assay Kit for Cyclic AMP Response Element Binding Protein (CREB)

CAT: 0338-TRV-060549Size: 96 TestsDry Ice: NoHazardous: No

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CAT#:0338-TRV-060549Size:96 Tests

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UniProt

Q01147

Applications

FLIA Kit for Antigen Detection.

Assay Principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Assay Protocol

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37° C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37° C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37° C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Assay Performance Time

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Detection Range

0.488 - 500 pg/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level of the target, etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level of the target, etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/meanX100, Intra-Assay: CV<10%, Inter-Assay: CV<12%

Sensitivity

The minimum detectable dose of this kit is typically less than 0.163pg/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of the target, etc. by FLIA (Flow Luminescence Immunoassay) .
No significant cross-reactivity or interference between the target, etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Method

Double-Antibody Sandwich

Organism Species

Mus musculus (Mouse)

UniProtKB · Q01147

Cyclic AMP-responsive element-binding protein 1

CREB1_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: Q01147
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Creb1
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: CREB-1, cAMP-responsive element-binding protein 1
EC number: —
Processing: —
Secondary accessions: —

Protein keywords

Technical term

3D-structureReference proteome

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicing

Biological process

Biological rhythmsDifferentiationTranscriptionTranscription regulation

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Cellular component

Nucleus

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Alternative Products

Catalog	Name
C7915-25	CREB (cAMP Response Element Binding Protein)
C7915-01R	CREB, phosphorylated (Ser133) (CBP, CREB binding protein, cAMP Response Element Binding Protein)
MBS6470624-01	CREB (cAMP Response Element Binding Protein) (APC)
MBS6259073-01	CREB (cAMP Response Element Binding Protein)-PE
abx134057-01	cAMP Response Element Binding Protein (CREB) Antibody
C7915-27-PE	CREB (cAMP Response Element Binding Protein) (PE)
C7915-27-HRP	CREB (cAMP Response Element Binding Protein) (HRP)
C7915-27-Biotin	CREB (cAMP Response Element Binding Protein) (Biotin)
C7915-02	CREB (cAMP Response Element Binding Protein) discontinued
MBS6258656-01	CREB (cAMP Response Element Binding Protein) (FITC)