Multiplex Assay Kit for Cyclophilin 40 (CYP-40), etc. by FLIA (Flow Luminescence Immunoassay)

• Product Name Alternative:

  PPID; CYPD; CyP-D; PPIase D; Peptidylprolyl Isomerase D; Cyclophilin 40; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-related protein; Rotamase D

• Synonyms:

  Cyclophilin 40

• UniProt:

  Q08752

• Applications:

  FLIA Kit for Antigen Detection.

• Field of Research:

  Signal transduction

• Assay Principle:

  Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

• Assay Protocol:

  1. Preparation of standards, reagents and samples before the experiment;
  2. Add 100μL standard or sample to each well,
          add 10μL magnetic beads, and incubate 90min at 37° C on shaker;
  3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37° C on shaker;
  4. Wash plate on magnetic frame for three times;
  5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37° C on shaker;
  6. Wash plate on magnetic frame for three times;
  7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
  

• Assay Performance Time:

  3H

• Sample Type:

  Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  0.02 - 20 ng/mL

• Precision:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cyclophilin 40 (CYP-40), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
  Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cyclophilin 40 (CYP-40), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
  CV (%) = SD/meanX100
  Intra-Assay: CV<10%
  Inter-Assay: CV<12%
  

• Sensitivity:

  The minimum detectable dose of this kit is typically less than 0.007 ng/mL

• Stability:

  The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
  To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Cyclophilin 40 (CYP-40), etc. by FLIA (Flow Luminescence Immunoassay) .
  No significant cross-reactivity or interference between Cyclophilin 40 (CYP-40), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

• Method:

  Double-Antibody Sandwich

• Organism Species:

  Homo sapiens (Human)