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ELISA Kit for Beta-2-Microglobulin (b2M)

CAT: 0338-TRV-047682-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-047682-01Size:24 Tests
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CAS Number
7732-18-5
Product Name Alternative
BMG; B2MG
UniProt
P01887
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Infection immunity; Kidney biomarker
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-2-Microglobulin (b2M) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-2-Microglobulin (b2M) and unlabeled Beta-2-Microglobulin (b2M) (Standards or samples) with the pre-coated antibody specific to Beta-2-Microglobulin (b2M) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-2-Microglobulin (b2M) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-2-Microglobulin (b2M) in the sample.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Assay Performance Time
2h
Sample Type
Serum, plasma, urine and other biological fluids
Detection Range
123.5-10,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 48.9pg/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Beta-2-Microglobulin (b2M) . No significant cross-reactivity or interference between Beta-2-Microglobulin (b2M) and analogues was observed.
Product Name Synonym
Beta-2-Microglobulin
Method
Competitive Inhibition
Organism Species
Mus musculus (Mouse)

UniProtKB · P01887

Beta-2-microglobulin

B2MG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01887
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
B2m
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q91XJ8
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Disulfide bond

Biological process

Immunity

Domain

Immunoglobulin domainSignal

Cellular component

MHC ISecreted

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