Products for Research Use Only
RegisterLogin

High Sensitivity ELISA Kit for Testosterone (Testo)

CAT: 0338-TRV-048713-01Size: 24 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0338-TRV-048713-01Size:24 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Product Name Alternative
T; Testesteron; Testeronun
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Testosterone (Testo) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Testosterone (Testo) and unlabeled High Sensitive Testosterone (Testo) (Standards or samples) with the pre-coated antibody specific to High Sensitive Testosterone (Testo) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Testosterone (Testo) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Testosterone (Testo) in the sample.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Assay Performance Time
3H
Sample Type
Serum, plasma and other biological fluids
Detection Range
2.47 - 200 pg/mL
Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Sensitivity
The minimum detectable dose of this kit is typically less than 0.93pg/mL
Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Testosterone (Testo) .
No significant cross-reactivity or interference between High Sensitive Testosterone (Testo) and analogues was observed.

Method
Competitive Inhibition
Organism Species
Pan-species (General)

Related Products

CatalogName
TRV-051786-01ELISA Kit for Testosterone (Testo)
TRV-048441-01CLIA Kit for Testosterone (Testo)
TRV-056138Rapid General Testosterone (Testo) ELISA Kit
TRV-053590-01Low Sample Volume ELISA Kit for Testosterone (Testo)
TRV-030539-01BSA Conjugated Testosterone (Testo)
TRV-021239-01Monoclonal Antibody to Testosterone (Testo)
TRV-011856-01Monoclonal Antibody to Testosterone (Testo)
TRV-021246-01Monoclonal Antibody to Testosterone (Testo)
TRV-021327-01Monoclonal Antibody to Testosterone (Testo)
TRV-017430-01Polyclonal Antibody to Testosterone (Testo)

Alternative Products

CatalogName
EKN48716Testosterone (Testo) ELISA Kit
EK250657Testo (Testosterone) ELISA Kit
19TESTO (Testosterone) ELISA test
KT-60838Rat Testosterone (TESTO) ELISA
MBS2700149-01General Testosterone (Testo) ELISA Kit
MBS8807480-01Horse Testo (Testosterone) ELISA Kit
MBS8821455-01Cattle Testo (Testosterone) ELISA Kit
EK251359Goat Testo (Testosterone) ELISA Kit
EK248386Mouse Testo (Testosterone) ELISA Kit
EK240279Chicken Testo (Testosterone) ELISA Kit

Popular Products