Products for Research Use Only
RegisterLogin

ELISA Kit for BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)

CAT: 0338-TRV-048778-01Size: 24 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0338-TRV-048778-01Size:24 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Product Name Alternative
C6.1A; BRCC36; C6.1A; CXorf53; Lys-63-specific deubiquitinase BRCC36; BRCA1-A complex subunit BRCC36; BRISC complex subunit BRCC36
UniProt
P46736
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Signal transduction
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.057ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) . No significant cross-reactivity or interference between BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) and analogues was observed.
Product Name Synonym
BRCA1/BRCA2 Containing Complex Subunit 3
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

UniProtKB · P46736

Lys-63-specific deubiquitinase BRCC36

BRCC3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P46736
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BRCC3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.19.-
Processing
—
Secondary accessions
A6QRF8, A6QRF9, A8MUX5, A8MWH0, A9Z1Y0, A9Z1Y5, B1B062, B4DQN7, Q16107, Q53YX5, Q9BTZ6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Biological process

Cell cycleCell divisionDNA damageDNA repairMitosisUbl conjugation pathway

Molecular function

Chromatin regulatorHydrolaseMetalloproteaseProtease

Cellular component

CytoplasmCytoskeletonNucleus

Ligand

Metal-bindingZinc

Disease

Proto-oncogene

Related Products

CatalogName
TRV-034764-01Recombinant BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
TRV-020890-01Monoclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
TRV-016734-01Polyclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
TRV-025235-01Polyclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
TRV-064126Multiplex Assay Kit for BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3), etc. by FLIA (Flow Luminescence Immunoassay)
TRV-046424-01ELISA Kit for Breast Cancer Susceptibility Protein 2 (BRCA2)
TRV-048043-01ELISA Kit for Breast Cancer Susceptibility Protein 2 (BRCA2)
TRV-054379-01ELISA Kit for Succinate Dehydrogenase Complex Subunit A (SDHA)
TRV-053550-01ELISA Kit for Breast Cancer Susceptibility Protein 1 (BRCA1)
TRV-046604-01ELISA Kit for Succinate Dehydrogenase Complex Subunit C (SDHC)

Alternative Products

CatalogName
FY-AB50346-01Anti-Brca1/Brca2 Containing Complex Subunit 3 (BRCC3)
MBS2033169-01Recombinant BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
CAU35636-01BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) Polyclonal Antibody
RPU50966-01Recombinant Human BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
EKU02801Human BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) ELISA Kit
EK246754Human BRCC3 (BRCA1/BRCA2 Containing Complex Subunit 3) ELISA Kit
KBH2075GENLISA™ Human BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) ELISA
MBS2084124-01HRP-Linked Polyclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
MBS2084123-01FITC-Linked Polyclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)
MBS2084120-01PE-Linked Polyclonal Antibody to BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3)

Popular Products