ELISA Kit for Complement Component 8b (C8b)

CAT: 0338-TRV-0041318-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0338-TRV-0041318-01Size:24 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
CAS Number
7732-18-5
Product Name Alternative
Complement component 8 subunit beta
UniProt
P07358
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Signal transduction; Immune molecule; Rheumatology
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 8b (C8b) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 8b (C8b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 8b (C8b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 8b (C8b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Sample Type
Plasma.
Detection Range
0.312-20ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 8b (C8b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 8b (C8b) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.128ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Component 8b (C8b) . No significant cross-reactivity or interference between Complement Component 8b (C8b) and analogues was observed.
Product Name Synonym
Complement Component 8b
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

Related Products

CatalogName
TRV-0012863-01Recombinant Complement Component 8b (C8b)
TRV-0005110-01Active Complement Component 8b (C8b)
TRV-0012923-01Recombinant Complement Component 8b (C8b)
TRV-0054020-01Recombinant Complement Component 8b (C8b)
TRV-0051030-01Polyclonal Antibody to Complement Component 8b (C8b)
TRV-0000278-01Polyclonal Antibody to Complement Component 8b (C8b)
TRV-0043670-01Polyclonal Antibody to Complement Component 8b (C8b)
TRV-0035779-01Monoclonal Antibody to Complement Component 8b (C8b)
TRV-0027879Multiplex Assay Kit for Complement Component 8b (C8b), etc. by FLIA (Flow Luminescence Immunoassay)
TRV-0063399-01ELISA Kit for Complement Component 3a (C3a)

Alternative Products

CatalogName
151685Complement Component 8b (C8b)
406159-01C8B (Complement Component C8 beta Chain, Complement Component 8 Subunit beta, C8B)
abx102223-01Complement Component 8b (C8b) Antibody
MBS2031818-01Recombinant Complement Component 8b (C8b)
FY-AB44927-01Anti-Complement Component 8B (C8B)
MBS2162356-01Active Complement Component 8b (C8b)
MBS646102-01C8B, CT (C8B, Complement component C8 beta chain, Complement component 8 subunit beta)
MBS8803881-01Human C8b (Complement Component 8b) ELISA Kit
MBS2703152-01Human Complement Component 8b (C8b) ELISA Kit
MBS2513906-01Monkey C8b (Complement Component 8b) ELISA Kit

Popular Products