CLIA Kit for Tryptase (TPS)

CAT: 0338-TRV-051261-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0338-TRV-051261-01Size:24 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

UniProt

Q15661

Applications

Chemiluminescent immunoassay for Antigen Detection.

Field of Research

Enzyme & Kinase; Infection immunity; Rheumatology

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Tryptase (TPS) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tryptase (TPS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tryptase (TPS) level in the sample or standard.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.

Assay Performance Time

2h, 40min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tryptase (TPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 0.61pg/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Tryptase (TPS) . No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.

Product Name Synonym

Tryptase

Method

Double-antibody Sandwich

Organism Species

Homo sapiens (Human)

UniProtKB · Q15661

Tryptase alpha/beta-1

TRYB1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q15661
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: TPSAB1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: Tryptase-1
EC number: 3.4.21.59
Processing: Precursor
Secondary accessions: D2E6R9, D2E6S1, P15157, Q15663, Q6B052, Q9H2Y4, Q9H2Y5, Q9UQI1

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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