ELISA Kit for Orexin (OX)

CAT: 0338-TRV-053974-01Size: 24 TestsDry Ice: NoHazardous: No

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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CAS Number

7732-18-5

Product Name Alternative

HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor

UniProt

O55241

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Neuro science

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to orexin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled orexin and unlabeled orexin (Standards or samples) with the pre-coated antibody specific to orexin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of orexin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of orexin in the sample.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
And then add 50µ L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.

Assay Performance Time

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

6.17-500pg/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Orexin (OX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Orexin (OX) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 2.13pg/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Orexin (OX) . No significant cross-reactivity or interference between Orexin (OX) and analogues was observed.

Product Name Synonym

Orexin

Method

Competitive Inhibition

Organism Species

Mus musculus (Mouse)

UniProtKB · O55241

Hypocretin neuropeptide precursor

OREX_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: O55241
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Hcrt
Protein existence: 2: Evidence at transcript level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

PTM

AmidationCleavage on pair of basic residuesDisulfide bondPyrrolidone carboxylic acid

Cellular component

Cytoplasmic vesicleEndoplasmic reticulumSynapse

Molecular function

Neuropeptide

Technical term

Reference proteome

Domain

Signal

Related Products

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