ELISA Kit for Mitoferrin (MFRN)

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CAS Number

7732-18-5

Product Name Alternative

SLC25A37; MSC; HT015; MSCP; Solute Carrier Family 25 Member 37; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein

UniProt

Q9NYZ2

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Tumor immunity

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitoferrin (MFRN) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitoferrin (MFRN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitoferrin (MFRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitoferrin (MFRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Assay Performance Time

Sample Type

Tissue homogenates and other biological fluids.

Detection Range

0.156-10ng/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mitoferrin (MFRN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mitoferrin (MFRN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 0.053ng/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Mitoferrin (MFRN) . No significant cross-reactivity or interference between Mitoferrin (MFRN) and analogues was observed.

Product Name Synonym

Mitoferrin

Method

Double-antibody Sandwich

Organism Species

Homo sapiens (Human)

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number	7732-18-5
PubChem CID	962
IUPAC Name	oxidane
Molecular Formula	H2O
Molecular Weight	18.015
XLogP	-0.5
Topological Polar Surface Area	1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Heavy Atom Count	1
Formal Charge	0
Complexity	0

SMILES

InChI

InChI=1S/H2O/h1H2

InChIKey

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Chemical Structure

2D Structure

CAS: 7732-18-5

CID: 962

Formula: H2O

MW: 18.015

Interactive 3D Structure

Loading 3D structure...

View on PubChem Raw 3D data

Computed Properties

Property	Value
Molecular Weight	18.015
XLogP3	-0.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Exact Mass	18.010564683
Monoisotopic Mass	18.010564683
Topological Polar Surface Area	1 Å²
Heavy Atom Count	1
Formal Charge	0
Complexity	0
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

UniProtKB · Q9NYZ2

Mitoferrin-1

MFRN1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q9NYZ2
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: SLC25A37
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: A2RU93, Q53FT7, Q69YJ8, Q969S1, Q9P0J2

Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Ion transportIron transportTransport

Ligand

Iron

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Technical term

Proteomics identificationReference proteome

Domain

RepeatTransmembraneTransmembrane helix

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