Products for Research Use Only
RegisterLogin

ELISA Kit for Integrin Alpha 2 (ITGa2)

CAT: 0338-TRV-051343-01Size: 24 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0338-TRV-051343-01Size:24 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Product Name Alternative
CD49b; BR; GPIa; VLA-2; VLAA2; Platelet membrane glycoprotein Ia; Alpha 2 Subunit Of VLA-2 Receptor; Very Late Activation Proteins 2 Receptor; Collagen receptor; VLA-2 alpha
UniProt
P17301
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Signal transduction; CD & Adhesion molecule; Tumor immunity
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 2 (ITGa2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 2 (ITGa2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 2 (ITGa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 2 (ITGa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Integrin Alpha 2 (ITGa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Integrin Alpha 2 (ITGa2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.060ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 2 (ITGa2) . No significant cross-reactivity or interference between Integrin Alpha 2 (ITGa2) and analogues was observed.
Product Name Synonym
Integrin Alpha 2
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

UniProtKB · P17301

Integrin alpha-2

ITA2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17301
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGA2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q14595
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesionHost-virus interaction

PTM

Disulfide bondGlycoprotein

Molecular function

Host cell receptor for virus entryIntegrinReceptor

Cellular component

Membrane

Domain

RepeatSignalTransmembraneTransmembrane helix

Related Products

CatalogName
TRV-056273Rat Integrin Alpha 2 (ITGa2) ELISA Kit
TRV-034579-01Recombinant Integrin Alpha 2 (ITGa2)
TRV-029126-01Recombinant Integrin Alpha 2 (ITGa2)
TRV-042740-01Active Integrin Alpha 2 (ITGa2)
TRV-041609-01Recombinant Integrin Alpha 2 (ITGa2)
TRV-043855-01Eukaryotic Integrin Alpha 2 (ITGa2)
TRV-037519-01Recombinant Integrin Alpha 2 (ITGa2)
TRV-034030-01Recombinant Integrin Alpha 2 (ITGa2)
TRV-029400-01Recombinant Integrin Alpha 2 (ITGa2)
TRV-008444-01Polyclonal Antibody to Integrin Alpha 2 (ITGa2)

Alternative Products

CatalogName
216740ITGA2 (Integrin alpha 2)
KLR4033GENLISA™ Integrin Alpha 2 (ITGa2) ELISA
MBS2030176-01Recombinant Integrin Alpha 2 (ITGa2)
abx005642-01Integrin Alpha 2 (ITGA2) Antibody
FY-AB51711-01Anti-Integrin Alpha 2 (ITGA2)
MBS2162351-01Active Integrin Alpha 2 (ITGa2)
abx421385-01Integrin Alpha-2 (ITGA2) Antibody

Popular Products