Products for Research Use Only
RegisterLogin

CLIA Kit for Interferon Alpha (IFNa)

CAT: 0338-TRV-046295-01Size: 24 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0338-TRV-046295-01Size:24 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Product Name Alternative
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
Applications
Chemiluminescent immunoassay for Antigen Detection.
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard.;
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time
2H, 40min
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.37 - 1000 pg/mL
Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Sensitivity
The minimum detectable dose of this kit is typically less than 0.61pg/mL
Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa) .
No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.

Method
Double-Antibody Sandwich
Organism Species
Rhesus monkey (Simian)

Related Products

CatalogName
TRV-050100-01ELISA Kit for Interferon Alpha (IFNa)
TRV-051788-01ELISA Kit for Interferon Alpha (IFNa)
TRV-052434-01ELISA Kit for Interferon Alpha (IFNa)
TRV-050856-01ELISA Kit for Interferon Alpha (IFNa)
TRV-051964-01ELISA Kit for Interferon Alpha (IFNa)
TRV-047254-01ELISA Kit for Interferon Alpha (IFNa)
TRV-051811-01ELISA Kit for Interferon Alpha (IFNa)
TRV-051374-01ELISA Kit for Interferon Alpha (IFNa)
TRV-057157ELISA Kit for Interferon Alpha (IFNa)
TRV-047505-01ELISA Kit for Interferon Alpha (IFNa)

Alternative Products

CatalogName
EKU09637Mouse Interferon Alpha (IFNa) CLIA Kit
EKN50744Bovine Interferon Alpha (IFNa) CLIA Kit
EKU09635Horse Interferon Alpha (IFNa) CLIA Kit
MBS2000582-01Porcine Interferon Alpha (IFNa) CLIA Kit
MBS2708508-01Human Interferon Alpha (IFNa) CLIA Kit
EKN50751Rat Interferon Alpha (IFNa) CLIA Kit
EKN50747Chicken Interferon Alpha (IFNa) CLIA Kit
EKU09634Dog Interferon Alpha (IFNa) CLIA Kit
EKN50750Pig Interferon Alpha (IFNa) CLIA Kit
EKN50752Rabbit Interferon Alpha (IFNa) CLIA Kit

Popular Products