CLIA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b)

• Product Name Alternative:

  FJHN; TCF2; HNF1beta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2, Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3

• Applications:

  Chemiluminescent immunoassay for Antigen Detection.

• Assay Principle:

  The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Beta (HNF1b) level in the sample or standard.;

• Assay Protocol:

  1. Prepare all reagents, samples and standards;
  2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
  3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
  4. Aspirate and wash 3 times;
  5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
  6. Aspirate and wash 5 times;
  7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
  8. Read RLU value immediately.
  

• Assay Performance Time:

  2H, 40min

• Sample Type:

  Tissue homogenates and other biological fluids

• Detection Range:

  13.7 - 10000 pg/mL

• Precision:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively.
  Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate.
  CV (%) = SD/meanX100
  Intra-Assay: CV<10%
  Inter-Assay: CV<12%
  

• Sensitivity:

  The minimum detectable dose of this kit is typically less than 6.3pg/mL

• Stability:

  The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
  To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Beta (HNF1b) .
  No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.

• Method:

  Double-Antibody Sandwich

• Organism Species:

  Rattus norvegicus (Rat)