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CLIA Kit for Complement Component 5a (C5a)

CAT: 0338-GTR04075733-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0338-GTR04075733-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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UniProt
P06684
Applications
Chemiluminescent immunoassay for Antigen Detection.
Field of Research
Infection immunity; Immune molecule; Autoimmunity
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a (C5a) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 5a (C5a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 5a (C5a) level in the sample or standard.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time
2h, 40min
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
27.4-20,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 5a (C5a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 13.1pg/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Component 5a (C5a) . No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
Product Name Synonym
Complement Component 5a
Method
Double-antibody Sandwich
Organism Species
Mus musculus (Mouse)

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