ELISA Kit for Complement Component 1, S Subcomponent (C1s)

CAT: 0338-TRV-051013-02Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0338-TRV-051013-02Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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CAS Number

7732-18-5

Product Name Alternative

C1 esterase

UniProt

P09871

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Signal transduction; Infection immunity; Immune molecule

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, S Subcomponent (C1s) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, S Subcomponent (C1s) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, S Subcomponent (C1s), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 1, S Subcomponent (C1s) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Assay Performance Time

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

3.12-200ng/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 1.29ng/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Complement Component 1, S Subcomponent (C1s) . No significant cross-reactivity or interference between Complement Component 1, S Subcomponent (C1s) and analogues was observed.

Product Name Synonym

Complement Component 1, S Subcomponent

Method

Double-antibody Sandwich

Organism Species

Homo sapiens (Human)

UniProtKB · P09871

Complement C1s subcomponent

C1S_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P09871
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: C1S
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 3.4.21.42
Processing: Precursor
Secondary accessions: D3DUT4, Q9UCU7, Q9UCU8, Q9UCU9, Q9UCV0, Q9UCV1, Q9UCV2, Q9UCV3, Q9UCV4, Q9UCV5, Q9UM14

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumMetal-binding

Biological process

Complement pathwayImmunityInnate immunity

Disease

Disease variantEhlers-Danlos syndrome

PTM

Disulfide bondGlycoproteinHydroxylation

Domain

EGF-like domainRepeatSignalSushi

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

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Alternative Products

Catalog	Name
124113	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S)
221222-01	C1S (C1 esterase, Complement C1s subComponent, Complement C1s subComponent Heavy Chain, Complement C1s subComponent Light Chain, Complement Component 1 s subComponent) discontinued
124113-APC	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) (APC)
124113-PE	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) (PE)
124113-Biotin	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) (Biotin)
MBS6371655-01	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) (HRP)
MBS6371654-01	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) (FITC)
052977	C1s (Complement Component 1, S Subcomponent)
MBS6371652-01	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) APC
MBS6371651-01	C1S (Complement C1s Subcomponent, C1 Esterase, Complement Component 1 Subcomponent S) (AP)