ELISA Kit for Major Histocompatibility Complex Class I E (MHCE)

CAT: 0338-TRV-047463-01Size: 24 TestsDry Ice: NoHazardous: No

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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CAS Number

7732-18-5

Product Name Alternative

HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E

UniProt

P13747

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Signal transduction; Tumor immunity; Infection immunity; Immune molecule; Developmental science

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I E (MHCE) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I E (MHCE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I E (MHCE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I E (MHCE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Assay Performance Time

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

6.25-400pg/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Major Histocompatibility Complex Class I E (MHCE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Major Histocompatibility Complex Class I E (MHCE) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 2.27pg/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Major Histocompatibility Complex Class I E (MHCE) . No significant cross-reactivity or interference between Major Histocompatibility Complex Class I E (MHCE) and analogues was observed.

Product Name Synonym

Major Histocompatibility Complex Class I E

Method

Double-antibody Sandwich

Organism Species

Homo sapiens (Human)

UniProtKB · P13747

HLA class I histocompatibility antigen, alpha chain E

HLAE_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P13747
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: HLA-E
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: E2G051, Q30169, Q6DU44, Q9BT83, Q9GIY7, Q9GIY8

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Adaptive immunityHost-virus interactionImmunityInnate immunity

Cellular component

Cell membraneGolgi apparatusMembraneMHC ISecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

SignalTransmembraneTransmembrane helix

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