ELISA Kit for Linker For Activation Of T-Cell (LAT)

• Product Name Alternative:

  LAT1; Pp36; Linker Of Activated T Cells; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein

• CAS Number:

  7732-18-5

• Applications:

  Enzyme-linked immunosorbent assay for Antigen Detection.

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Linker For Activation Of T-Cell (LAT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Linker For Activation Of T-Cell (LAT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Linker For Activation Of T-Cell (LAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Linker For Activation Of T-Cell (LAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  1. Prepare all reagents, samples and standards;
  2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
  3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
  4. Aspirate and wash 3 times;
  5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
  6. Aspirate and wash 5 times;
  7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
  8. Add 50µ L Stop Solution. Read at 450nm immediately.
  

• Assay Performance Time:

  3H

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.312 - 20 ng/mL

• Precision:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Linker For Activation Of T-Cell (LAT) were tested 20 times on one plate, respectively.
  Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Linker For Activation Of T-Cell (LAT) were tested on 3 different plates, 8 replicates in each plate.
  CV (%) = SD/meanX100
  Intra-Assay: CV<10%
  Inter-Assay: CV<12%
  

• Sensitivity:

  The minimum detectable dose of this kit is typically less than 0.102ng/mL

• Stability:

  The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
  To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Linker For Activation Of T-Cell (LAT) .
  No significant cross-reactivity or interference between Linker For Activation Of T-Cell (LAT) and analogues was observed.

• Method:

  Double-Antibody Sandwich

• Organism Species:

  Homo sapiens (Human)