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ELISA Kit for Complement C4-B (C4B)

CAT: 0338-TRV-053350-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-053350-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Product Name Alternative
CO4; CPAMD3; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Complement component 4B, Chido blood group
UniProt
P0C0L5
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Metabolic pathway; Infection immunity; Immune molecule
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement C4-B (C4B) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement C4-B (C4B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement C4-B (C4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement C4-B (C4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.25-80ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement C4-B (C4B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement C4-B (C4B) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.55ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement C4-B (C4B) . No significant cross-reactivity or interference between Complement C4-B (C4B) and analogues was observed.
Product Name Synonym
Complement C4-B
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

UniProtKB · P0C0L5

Complement C4-B

CO4B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P0C0L5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C4B, C4B_2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A2BHY4, P01028, P78445, Q13160, Q13906, Q14033, Q14835, Q6U2E9, Q6U2G1, Q6U2I5, Q6U2L1, Q6U2L7, Q6U2L9, Q6U2M5, Q6VCV8, Q96SA7, Q9NPK5, Q9UIP5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

Blood group antigen

Cellular component

Cell projectionSecretedSynapse

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinSulfationThioester bond

Biological process

Complement pathwayImmunityInflammatory responseInnate immunity

Domain

Signal

Disease

Systemic lupus erythematosus

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