Recombinant Human Glucose-6-phosphatase (G6PC) , partial

Recombinant Human Glucose-6-phosphatase (G6PC) , partial

• CAS Number: 9000-83-3
• Gene Name: G6PC1
• UniProt: P35575
• Expression Region: 82-117aa
• Organism: Homo sapiens
• Target Sequence: QRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPS
• Tag: N-terminal 6xHis-sumostar-tagged
• Source: Yeast
• Field of Research: Cancer
• Assay Type: In Stock Protein
• Relevance: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 17.5 kDa
• References & Citations: "Identification of mutations in the glucose-6-phosphatase gene in Czech and Slovak patients with glycogen storage disease type Ia, including novel mutations K76N, V166A and 540del5." Kozak L., Francova H., Hrabincova E., Stastna S., Peskova K., Elleder M. Hum. Mutat. 16:89-89 (2000).
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.