MMP9 (Cleaved-Phe107) rabbit pAb

MMP9 (Cleaved-Phe107) rabbit pAb

• Description: Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V. Cofactor: Binds 2 zinc ions per subunit. Cofactor: Binds 3 calcium ions per subunit. Disease: Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM: 603932]. LDH is the predominant cause of low-back pain and unilateral leg pain. Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. enzyme regulation: Inhibited by histatin-3 1/24 (histatin-5). function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. induction: Activated by 4-aminophenylmercuric acetate and phorbol ester. miscellaneous: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status. PTM: N- and O-glycosylated. PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. similarity: Belongs to the peptidase M10A family. similarity: Contains 3 fibronectin type-II domains. similarity: Contains 4 hemopexin-like domains. subunit: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. tissue specificity: Produced by normal alveolar macrophages and granulocytes.
• Synonyms: Matrix metalloproteinase-9 (MMP-9;EC 3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB) [Cleaved into: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9]
• Gene ID: 4318
• UniProt: P14780
• Cellular Locus: Secreted, extracellular space, extracellular matrix.
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: Synthesized peptide derived from human MMP9 (Cleaved-Phe107)
• Clonality: Polyclonal
• Validated Applications: WB, IHC
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:500-2000;IHC-p 1:50-300
• Molecular Weight: 66 78kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.