CAMP ELISA KIT

• Background :

  Adenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors (GPCRs) . Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody (cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50 (50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL.

• Specifications :

  CAMP

• Detection Method :

  Colorimetric

• Assay Type :

  Quantitative

• Sample Type :

  Plasma, Serum

• Detection Range :

  1.56-200 pmol/mL

• Recovery :

  80-120%

• Sensitivity :

  1.56 pmol/mL

• Shipping Conditions :

  2-8 °C

• Storage Conditions :

  The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.

• Notes :

  For research use only.