Recombinant Mouse IL-12B (p40) Protein

• Background:

  Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

• Abbreviation:

  IL-12B

• Synonyms:

  NKSF; IL-12/IL-23 p; IL-12 subunit p; NK cell stimulatory factor chain; NK cell Stimulating Factor Chain; CLMF; CLMF p; IL12 p; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; Cytotoxic Lymphocyte Maturation Factor 40 kDa subunit (CLMF p40) ; IL12; IL-12 p40; IL12 p70; IL-12 p70; IL-12/IL-23 p40; NK cell Stimulating Factor Chain 2; p40; IL-12B; NKSF2; Interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; IL 12B; Il12b

• UniProt:

  P43432

• Accession Number:

  P43432

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met1-Ser335

• Field of Research:

  Cancer; Cardiovascular; Immunology;

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  37.0 kDa

• Observed Molecular Weight:

  40 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse