Recombinant Mouse HSP-70/HSPA9 protein (His tag)

• Background:

  Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU.Regulates erythropoiesis via stabilization of ISC assembly.May play a role in the control of cell proliferation and cellular aging.

• Abbreviation:

  HSP-70, HSPA9

• Synonyms:

  75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; MOT; Mortalin; PBP74; Peptide-binding protein 74

• UniProt:

  P38647

• Accession Number:

  P38647

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Glu 542-Gln 679

• Field of Research:

  Cancer; Metabolism; Neuroscience; Sgnal Transduction

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  15.1 kDa

• Observed Molecular Weight:

  20 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse