Recombinant Human Fibronectin protein (GST, His tag)

• Background:

  Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Similar to integrins, fibronectin binds extracellular matrix components such as collagen, fibrin, and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

• Abbreviation:

  Fibronectin

• Synonyms:

  CIG; ED-B; FINC; FN; FN1; FNZ; Fibronectin; GFND; GFND2; LETS; MSF

• UniProt:

  P02751

• Accession Number:

  P02751

• Expression System:

  E.coli

• Tag:

  N-GST & C-His

• Sequence:

  Gln 32-Arg 230

• Field of Research:

  Signal Transduction; Cardiovascular; Cancer; Developmental Biology; Stem cells

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  46.8 kDa

• Observed Molecular Weight:

  50 kDa

• Species:

  Human