Recombinant Human MEF-2C protein (His tag)

• Background:

  MEF2C (Myocyte enhancer factor-2C) is a transcriptional activator that is a member of the MEF2 subfamily, MADS (MCM1, Agamous, Deficiens, Serum response factor) gene family of proteins. Although its predicted MW is 51 kDa, it runs anomalously at 57 kDa in SDS-PAGE. It is expressed in B cells, plus postmitotic neurons and skeletal muscle cells that are undergoing differentiation.

• Abbreviation:

  MEF-2C

• Synonyms:

  MEF2C; Myocyte-specific enhancer factor 2C

• UniProt:

  Q06413

• Accession Number:

  Q06413-1

• Expression System:

  E. coli

• Tag:

  N-His & C-His

• Sequence:

  Pro 101-Trp 350

• Field of Research:

  Cardiovascular; Neuroscience; Signal Transduction; Stem Cells

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  27.4 kDa

• Observed Molecular Weight:

  32 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human