Recombinant Human AIF1 Protein (His Tag)

• Background:

  Allograft Inflammatory Factor 1 (AIF1) contains two EF-hand domains and exists as a homodimer. AIF1 can be detected in T-lymphocytes and peripheral blood mononuclear cells. AIF1 functions as actin-binding protein that enhances membrane ruffling and RAC activation and can enhance the actin-bundling activity of LCP1. In addition, AIF1 plays a role in RAC signaling and in phagocytosis and may also in macrophage activation and function. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes and plays a role in vascular inflammation.

• Abbreviation:

  AIF1

• Synonyms:

  AIF-1; AIF1; Allograft Inflammatory Factor 1; G1; IBA1; Ionized Calcium-Binding Adapter Molecule 1; Protein G1

• UniProt:

  P55008

• Accession Number:

  P55008

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Ser2-Pro147

• Field of Research:

  Cell biology; Neuroscience; immunology; metabolism

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.7 kDa

• Observed Molecular Weight:

  16 kDa

• Species:

  Human