Recombinant Mouse Fetuin A/AHSG Protein (Fc Tag)

• Background:

  Fetuin-A , also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family , is a plasma binding protein , and is more abundant in fetal than adult blood. It is involved in several functions , such as endocytosis , brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A , a well-described inhibitor of calcification , regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However , high circulating fetuin-A levels appear to induce insulin resistance and , in non-dialyzed subjects with diabetic nephropathy , are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally , Human fetuin-A is a negative acute phase protein involved in inflammatory diseases , thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

• Abbreviation:

  Fetuin A/AHSG

• Synonyms:

  KUB; SGP; TRPM; NA1/NA; A2HS; AHS; alpha-2-HS-glycoprotein; APOJ; APO-J; CLI; Countertrypin; Fetuin-A; HSGA; KUB1; NA1/NA2; SGP2; SP-40; TRPM2; Fetua; Ahsg

• UniProt:

  P29699

• Accession Number:

  P29699

• Expression System:

  Mammalian

• Tag:

  C-Fc

• Sequence:

  Ala19-Ile345

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  57.6 kDa

• Observed Molecular Weight:

  70-80 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse