Recombinant Human CCL18/PARC Protein (Trx Tag)

• Background:

  CCL18/PARC is a chemotactic cytokine involved in the pathogenesis and progression of various disorders, including cancer. Proof showed high levels of CCL18/PARC in the serum of epithelial ovarian carcinoma patients sμggesting its potential as a circulating biomarker. CCL18/PARC chemokine has an important role in chemokine-mediated tumor metastasis, and may serve as a potential predictor for poor survival outcomes for ovarian cancer. (CCL18/PARC) is predominantly secreted by M2-tumor associated macrophages (TAMs) and promotes malignant behaviors of various human cancer types. CCL18/PARC has a correlation with cardiac function in patients with AAMI and it might be considered as an indicator of poor LVEF in patients with AAMI. Circulating and WAT-secreted CCL18/PARC correlates with insulin resistance and metabolic risk score. Because CCL18/PARC is macrophage-specific and associates with adipose immune gene expression, it may constitute a marker of WAT inflammation. Macrophages are thoμght to be the main source of CCL18/PARC, and the effect of pirfenidone, an anti-fibrotic agent for idiopathic pulmonary fibrosis, on the expression of CCL18/PARC in macrophages warrants investigation.

• Abbreviation:

  CCL18/PARC

• Synonyms:

  CKb; TSC; DCCK; DC-CK; Eotaxin; AMAC-1; CCL26; CKb7; DC-CK1; Eotaxin-3; IMAC; MIP-4; MIP-4a; SCYA26; TSC-1; AMAC1; MIP4; PARC; SCYA18; CCL18; AMAC-1; CKb7; DCCK1; DC-CK1; MIP-4; Alternative macrophage activation associated CC chemokine 1; Alternative macrophage activation-associated CC chemokine 1; CC chemokine PARC; C-C Motif Chemokine 18; CCL18 (4-69) ; Dendritic cell chemokine 1; Macrophage inflammatory protein 4; Pulmonary and activation regulated chemokine; Pulmonary and activation-regulated chemokine; Small inducible cytokine A18; Small-inducible cytokine A18

• UniProt:

  P55774

• Accession Number:

  P55774

• Expression System:

  E.coli

• Tag:

  N-Trx

• Sequence:

  Ala21-Ala89

• Field of Research:

  Immunology

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  27.4 kDa

• Observed Molecular Weight:

  30 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Human