Recombinant Human DKK-4 Protein (Trx Tag)

• Background:

  This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2.

• Abbreviation:

  DKK-4

• Synonyms:

  Dkk; DKK4; Dickkopf-4; hDkk-4

• UniProt:

  Q9UBT3

• Accession Number:

  Q9UBT3

• Expression System:

  E. coli

• Tag:

  N-Trx

• Sequence:

  Leu19-Leu224

• Field of Research:

  Stem Cells

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  42.5 kDa

• Observed Molecular Weight:

  43 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Human