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High Sensitivity Mouse C3 (Complement Component 3) ELISA Kit

CAT: 0855-ELK11082HS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11082HS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein
Reactivity
Mouse
Field of Research
Signal transduction; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
400 pg/mL
Sample Type
Serum, plasma
Detection Range
6.25-400 pg/mL
Sensitivity
2.2 pg/mL
Uniprot Accession Number
P01027

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