Rabies virus (strain ERA) Nucleoprotein, His Tag

• Background:

  Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L) . Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation.

• Description:

  Rabies virus (strain ERA) Nucleoprotein, His Tag (NUN-R55H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ser 450 (Accession # P0DOF3) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.6 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P0DOF3

• Expression Region:

  Met 1 - Ser 450

• Host:

  Insect Cells

• Target:

  Nucleoprotein/NP (RABV)

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Rabies virus

• Stability:

  ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Rabies virus (strain ERA) Nucleoprotein, His Tag (Cat. No. NUN-R55H3) at 5 μg/mL (100 μL/well) can bind Rabies Virus Nucleoprotein (RABV N) Antibody (Biotin) with a linear range of 0.156-5 μg/mL (QC tested).

• Format:

  Liquid

• Buffer:

  50 mM Tris, 500 mM NaCl, pH7.5

• Additives:

  Glycerol

• Molecular Weight:

  52.6 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  1mg*1

• Species:

  Rabies virus