Anti-HCN4 Cyclic nucleotide-gated channel Antibody

Anti-HCN4 Cyclic nucleotide-gated channel Antibody

• Description: Mouse Monoclonal Antibody specific to HCN4 Cyclic nucleotide-gated Channel
• Product Name Alternative: Anti-HCN4 Cyclic nucleotide-gated Channel Mouse Monoclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: Hcn4
• NCBI Gene ID: Hcn4
• UniProt: Q9JKA7
• Accession Number: NP_067690.1
• Cellular Locus: Cell membrane, Multi-pass membrane protein
• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein aa 1019-1108 (cytoplasmic C-terminus) of rat HCN4 (accession no. Q9JKA7).
• Target Antigen: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• Target: HCN4 Cyclic nucleotide-gated channel
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, IHC, ICC/IF, AM, FACS
• Field of Research: Ion Channels
• Purification Method: Purified by Protein G affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium which is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih) (By similarity). May mediate responses to sour stimuli. {PubMed:11675786}.
• Additionnal Information: Immunoblotting: use at 1-10ug/mL. A band of ~1300kDa is detected.Immunohistochemistry and
  
  Immunocytochemistry: use at 0.1-1ug/mL
  
  Immunofluorescence: use at 1-10ug/mL
  
  These are recommended concentrations. User should determine optimal concentrations for their application.
  
  Positive control: COS cell lysate transiently transfected with HCN4.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody recognizes human, mouse, and rat HCN4. It does not cross-react with other HCNs.
• Formulation: PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
• Buffer pH: pH 7.4
• Target Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN4, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain.