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Recombinant Human NEDD8 Protein

CAT: 1676-PR01909Size: 10μg/50μg/500μg/1mgDry Ice: NoHazardous: No
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CAT#:1676-PR01909Size:10μg/50μg/500μg/1mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Product Name Alternative
Neural precursor cell expressed developmentally down-regulated protein 8; NEDD8; Neddylin; Ubiquitin-like protein Nedd8
Accession Number
Q15843
Tag
Tag free
Source
E.coli
Endotoxin
<1 EU/μg as determined by LAL test.
Purity
>95% as determined by reducing SDS-PAGE.
Form
Lyophilized
Reconstitution
Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 μg/mL.
Molecular Weight
8.6 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. Please minimize freeze-thaw cycles and use a manual defrost freezer.
Protein Length
Met1-Gly76
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, pH 8.0.
Species
Human

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