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Recombinant K. pneumoniae NEO

CAT: 1676-PR02392Size: 10μg/50μg/500μg/1mgDry Ice: NoHazardous: No
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CAT#:1676-PR02392Size:10μg/50μg/500μg/1mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Aminoglycoside 3'-phosphotransferase (APH (3') ), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH (3') is primarily found in certain species of gram-positive bacteria.
Product Name Alternative
Aminoglycoside 3'-phosphotransferase; APH (3') -II; APH (3') II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
Accession Number
P00552
Tag
Tag free
Source
E.coli
Endotoxin
<1 EU/μg as determined by LAL test.
Purity
>95% as determined by reducing SDS-PAGE.
Form
Liquid
Molecular Weight
29 KDa
Shipping Conditions
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles and use a manual defrost freezer.
Protein Length
Met1-Phe264
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween80, PH8.0.
Species
Klebsiella pneumoniae

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