Recombinant Human TSLP Protein

Thymic stromal lymphopoietin (TSLP) is a novel member of the hemopoietic cytokine family that promotes the development of B cells and shares overlapping activity with IL-7. The human TSLP protein comprises a 28 amino acids (aa) signal sequence and 131 aa mature region. Human TSLP has two isoforms lfTSLP and sfTSLP produced by alternative splicing . lfTSLP is expressed in a number of tissues including heart, liver and prostate, and sfTSLP (63aa) is predominantly expressed in keratinocytes of oral mucosa, skin and in salivary glands. In aa sequence level, Human TSLP displays about 43% identity with mouse TSLP.TSLP is a cytokine that functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c (+) dendritic cells.TSLP has proliferative effects on the myeloid cell line and may initiate asthma or atopic dermatitis responses by directly activating mast cells . TSLP signals cells via the interleukin-7 receptor-α chain (IL-7Rα), shared with IL-7, together with the TSLP receptor (TSLPR) subunit. Recent studies indicate that TSLP and its receptor are novel therapeutic targets for rheumatoid arthritis, for increased intraarticular TSLP concentrations in patients has caused chemotaxis and activation of arthritogenic T cells.

Thymic stromal lymphopoietin; TSLP

Q969D9

Tag free

E.coli

<1 EU/μg as determined by LAL test.

>95% as determined by reducing SDS-PAGE.

Lyophilized

Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 μg/mL.

15.1 KDa

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. Please minimize freeze-thaw cycles and use a manual defrost freezer.

Tyr29-Gln159

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.