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Human TNF-α (Tumor Necrosis Factor Alpha) solid ELISPOT Kit

CAT: 0763-ESP-H0010S-01Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0763-ESP-H0010S-01Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
TNF-α
UniProt
P01375
Cross Reactivity
This kit recognizes Human TNF-α in samples.No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
Target
DIF, TNF-alpha, TNFA, TNFSF2
Applications
ELISPOT
Field of Research
Cell Biology; Immunology
Assay Principle
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human TNF-α antibodies. During the experiment, cell suspension and stimulants are added first, and TNF-α secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human TNF-α antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human TNF-α antibodies will bind specifically to the TNF-α which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human TNF-α secreting cells.
Assay Performance Time
3 h 30 min
Sample Type
Suspension cell
Precision
Both intra-CV and inter-CV are < 10%.
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Human
Recommended Usage
This ELISPOT kit is designed for the detection of the frequency of human TNF-α secreting cells.

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