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ADAR1 (E1008Q) FLAG-tag Recombinant

CAT: 0421-102535-1Size: 25 µgDry Ice: YesHazardous: No
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CAT#:0421-102535-1Size:25 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ADAR1 (adenosine deaminase, RNA-specific 1) performs adenosine to inosine base editing in RNA, particularly targeting adenosines located within a specific stem-loop motif structure. It is proposed that ADARs evolved to provide additional diversity to the transcriptome and while the majority of ADAR editing events occur in non-coding RNAs, some, including the canonical GluA2 editing site, alter the amino acid sequence of coding proteins. ADAR1 plays a role in innate immunity by mitigating interferon signaling. Dysfunction of ADAR1 results in autoimmune disorders, and impacts cancer cell growth and proliferation as well as tumor response to immunotherapy. Since ADAR recognizes double-stranded RNA, it also functions to suppress or modify RNA viruses. Thus, it is implicated in viral evolution and in the emergence of viral variants such as SARS-CoV-2 variants. The E1008Q mutant of ADAR1 has been proposed to demonstrate higher editing activity than its wild type, with the mutation being present in a highly conserved glutamate present in the deaminase domain of the protein.
Description
Recombinant human full-length ADAR1 (adenosine deaminase, RNA-specific 1) transcript 1, encompassing amino acids 2-1226 (end) . This protein contains the mutation of interest E1008Q. This construct contains an N-terminal FLAG-tag. The recombinant protein was affinity purified.
UniProt
P55265
Applications
Useful for the study of binding of ADAR1 and RNA and screening inhibitors of this interaction.
Purity
≥ 80%
Format
Aqueous buffer solution
Shipping Conditions
-80°C
Storage Conditions
> 6 months at –80°C.
Calculated Molecular Weight
137 kDa
Formulation
50 mM Tris-HCl, pH 8.0,750 mM NaCl, 0.01% Triton X-100,10% glycerol and 100 μg/ml FLAG peptide.

UniProtKB · P55265

Double-stranded RNA-specific adenosine deaminase

DSRAD_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P55265
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ADAR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
DRADA
EC number
3.5.4.37
Processing
—
Secondary accessions
B1AQQ9, B1AQR0, D3DV76, O15223, O43859, O43860, Q9BYM3, Q9BYM4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Aicardi-Goutieres syndromeDisease variant

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

Antiviral defenseImmunityInnate immunitymRNA processingRNA-mediated gene silencing

Cellular component

CytoplasmNucleus

Molecular function

DNA-bindingHydrolaseRNA-binding

PTM

Isopeptide bondMethylationPhosphoproteinUbl conjugation

Ligand

Metal-bindingZinc

Domain

Repeat

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