Anti-GPRC5D-Anti-CD3 Bispecific Antibody

• Background:

  The  orphan G protein–coupled receptor, class C group 5 member D (GPRC5D) is a retinoic acid-inducible protein with seven transmembrane segments and a short N-terminal extracellular region. The strong and selective expression of GPRC5D in Multiple Myeloma (MM) cells, similar to the expression pattern of B-cell maturation antigen (BCMA), makes it a promising target for the treatment of MM. It is anticipated that GPRC5D will be an alternative CAR (chimeric antigen receptor) -T cell target for MM immunotherapy.

• Description:

  Anti-GPRC5D-Anti-CD3 bispecific antibody is a purified recombinant human bispecific antibody with T cell engager. This bispecific antibody has been tested in a functional assay using NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #60621) in the presence of GPRC5D CHO Recombinant Cell Line (BPS Bioscience #78337) that overexpress GPRC5D (G protein–coupled receptor, class C group 5 member D) .

• Applications:

  Anti-GPRC5D-Anti-CD3 Bispecific Antibody can be used for studying GPRC5D+ cancer cell-mediated T cell activation, using either primary T cells or reporter cell lines such NFAT Luciferase Reporter Jurkat Cell Line (#60621) .

• Purification:

  Protein A affinity purification from HEK293 cells

• Concentration:

  1.62 mg/mL

• Endotoxin:

  0.9 EU/mg

• Purity:

  ≥ 90%

• Format:

  Aqueous buffer solution.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  Stable for at least 12 months at -80°C.

• Calculated Molecular Weight:

  Heavy Chain: 49 kDa + glycans; Light Chain: 24 kDa

• Formulation:

  8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol

• Glycosylation:

  This antibody runs at a higher MW by SDS-PAGE due to glycosylation.