KRAS (G12C), Isoform A, His-Tag, GDP-Loaded Recombinant

• Description:

  Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end) . In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease#50308) . The protein also contains cancer-associated mutation G12C. The protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column. Ready for use in the GDP/GTP exchange assay or as a negative control for KRAS-RAF interaction.

• UniProt:

  P01116

• Tag:

  N-terminal His-tag

• Applications:

  Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 90%

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  23 kDa

• Formulation:

  20 mM HEPES, pH 7.4,150 mM NaCl, and 1 mM DTT.