Chemi-Verse™ PI3 Kinase (p120γ) Kinase Assay Kit

• Background:

  PI3 (phosphoinositide 3) kinase, or phosphatidylinositol 3 kinases, are a family of proteins that can be subdivided into four classes: I, II, III and IV. Class I are involved in converting PI (4,5) P2 (phosphatidylinositol (4,5) -biphosphate) into PI (3,4, 5) P3 (phosphatidylinositol (3,4, 5) -triphosphate) when activated by tyrosine kinase receptors and G-protein coupled receptors. p120γ is also known as PIK3CG (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit), PI3Kγ or p110γ and belongs to class IB. p120γ plays a role in chemotaxis, cytokine release and ROS (reactive oxygen species) generation and is thus an important player in the innate and adaptive immune systems. It has been linked to inflammatory diseases, such as RA (rheumatoid arthritis), atherosclerosis and other cardiovascular pathologies, lupus and cancer. The use of p120γ inhibitors combined with immune checkpoints inhibitors has potential in cancer therapy. Further studies into the modes of action and structure of p120γ will allow the development of more selective inhibitors and advance the treatment of inflammatory diseases and cancer linked to this protein.

• Description:

  The Chemi-Verse™ PI3 Kinase (p120γ) Kinase Assay Kit is designed to measure PI3 (phosphoinositide 3) kinase (p120γ) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PI3 kinase (p120γ), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.Need us to run inhibitor screens or profile your compounds against this kinase? Check out ourKinase Screening and Profiling Services.

• UniProt:

  P48736

• Applications:

  Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput screening (HTS) applications.

• Format:

  Catalog #NameAmountStorage40625PI3 kinase (p120γ), His-Tag*2.5 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79386500 µM ATP50 µl-20°C40560PI3K Lipid Substrate (Packaged separately, Do Not Freeze!) 500 µl4°C79696White 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  The final concentration of DMSO in the assay should not exceed 1%.ThePI3K lipid substrate is shipped separately on ice. Please store it at 4°C upon arrival (DO NOT FREEZE the PI3K lipid substrate) .